Single-Electrode High-Throughput 12-Well Array Electrochemiluminescence Imaging Sensor for Portable Parallel Dual-Color Analysis of Dual Breast Cancer Susceptibility Genes

Rapid, high-throughput analysis of breast cancer susceptibility genes is essential in a clinical diagnosis. Herein, we designed a 12-well single-electrode electrochemiluminescence (ECL) imaging array biosensor for parallel high-throughput dual-color analysis of dual cancer genes (BRCA1 and BRCA2). Two newly explored luminophores, the Eu-luminol complex and Ru/Zn MOF with blue and orange-red high-intensity ECL emission, were introduced to the array, achieving high-resolution color-distinguishing ECL imaging of double targets. This work innovatively changed the fabrication technology of single-electrode arrays by replacing poly(dimethylsiloxane) (PDMS) with polypropylene tubes. This reformation enabled independent construction of 12 microcells, thus enhancing their stability and reducing production costs. The 12 microcells on the ITO electrode drastically reduce the target detection time and streamline the procedure compared to traditional three-electrode systems. The visual ECL signals were conveniently captured by using a smartphone, yielding readily distinguishable orange-red and blue images. The target-induced walker amplification technology is utilized to integrate the quenching probes Au@Ag NPs and CuS@Au NPs into the single electrode, enabling simultaneous ECL imaging of 12 microwells for detection of multiple targets. Owing to multiple outstanding advantages of high-throughput, fast speed, and convenience, the developed 12-well single-electrode dual-color ECL imaging biosensor holds considerable promise for broad applications in biological and environmental monitoring.