With the rapid development of omics technologies, metabolomics and lipidomics have become important tools for elucidating the molecular basis of reproductive performance in livestock. These approaches, which focus on small metabolites and lipid species, provide valuable insights into dynamic interactions between genes, proteins, and the environment, offering a high level of sensitivity. This review summarizes key methodological advances in metabolomics and lipidomics and their applications to gametogenesis, oocyte maturation, embryo development, and pregnancy maintenance. It highlights how metabolic and lipid pathways, particularly those involving energy metabolism, redox regulation, lipid remodeling, and cell–cell signaling, affect reproductive cell quality. This review further integrates biomarkers identified from semen, follicular fluid, oocytes, embryos, and uterine tissues, highlighting their potential roles in fertility assessment. Together, these insights enhance our understanding of metabolic regulation in reproduction and support the effective application of metabolomics and lipidomics in improving livestock fertility.
Sheng et al. (Thu,) studied this question.