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February 19, 2026Clinical Cancer Research

Abstract RF3-02: Gene Expression-based Subtyping of Early Triple-Negative Breast Cancer (TNBC) for Prediction of Response to Neoadjuvant Immune-chemotherapy in the NSABP B-59/GBG-96-GeparDouze Trial

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Authors

CDC. DenkertSRSivaramakrishna RachakondaTKThomas Karn

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Overview

This analysis reveals gene expression subtypes predict response to neoadjuvant immune-chemotherapy in triple-negative breast cancer, suggesting implications for treatment strategies.

Key Points

  • To evaluate gene expression subtyping of early triple-negative breast cancer (TNBC) for predicting responses to neoadjuvant immune-chemotherapy.
  • Analyzed 494 pre-therapeutic biopsies for 2549 genes using the HTG EdgeSeq system.
  • Employed classical AIMS and TNBC subtyping methods.
  • Endpoints included pathological complete response (pCR) and event-free survival (EFS).
  • Assessed predefined gene expression signatures focusing on immune, proliferation, and stromal genes.
  • Classical AIMS subtyping identified 70.5% Basal-like and 23.8% HER2-enriched subtypes.
  • TNBC subcategories included immunomodulatory (25.5%) and Mesenchymal (23.9%).
  • pCR rates varied by subtype, with IM subtype achieving 74.6% pCR.
  • Significant immune gene expression correlated with pCR and EFS in treated patients.

Cite This Study

Denkert et al. (2026) studied this question.

synapsesocial.com/papers/6996a869ecb39a600b3ef1e3https://doi.org/10.1158/1557-3265.sabcs25-rf3-02
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