The aim is to create a targeted probe for detecting O-GlcNAcase in biological samples.
Developed a glycoprobe using human serum albumin.
Tested the probe in cell lysates and live cells.
Compared detection efficacy between O-GlcNAcase and hexosaminidase.
The probe selectively detected O-GlcNAcase with minimal interference from hexosaminidase.
Effective detection observed in both cell lysates and live cells.
Abstract
A human serum albumin (HSA) encapsulated fluorogenic glycoprobe was developed for the selective detection of O -GlcNAcase (OGA) over hexosaminidase (HEX) in cell lysates and live cells.