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February 21, 2026Biophysical Journal0 citations

G-quadruplex Structures in SHMT1 5'UTR Impacts Methylation in Multiple Sclerosis

BPS2026 – Characterization of G-quadruplex structures in the SHMT1 5'UTR: Implications for regulating methylation in multiple sclerosis

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Authors

RPRosalia PalumboMKManju KasajuSHSophia C. Hershey

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Overview

Characterization of G-quadruplex in SHMT1 shows its role in methylation regulation in multiple sclerosis, suggesting new therapeutic targets.

Key Points

  • This research aims to characterize G-quadruplex structures in the SHMT1 gene and evaluate their impact on gene expression and methylation in multiple sclerosis.
  • Characterized G-quadruplex and i-motif structures using native polyacrylamide gel electrophoresis and circular dichroism spectroscopy.
  • Used proton nuclear magnetic resonance and ultraviolet thermal denaturation spectroscopy for structural analysis.
  • Performed luciferase reporter assays to evaluate the effect of G-quadruplex on protein expression.
  • Identified G-quadruplex and i-motif structures in SHMT1 DNA and G-quadruplex in its mRNA.
  • Found that G-quadruplex formation inhibits SHMT1 protein expression.
  • Proposed that targeting G-quadruplex may serve as a therapeutic approach for managing methylation errors in multiple sclerosis.
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Cite This Study

Palumbo et al. (2026) studied this question.

synapsesocial.com/papers/69990de85b97ab4c14ac2863https://doi.org/10.1016/j.bpj.2025.11.1812
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