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February 21, 2026Biophysical Journal0 citations

Torsion Regulates DNA Replication Stalling and Reactivation via Replisome Dynamics

BPS2026 – Torsion is a dynamic regulator of DNA replication stalling and reactivation

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Authors

XJXiaomeng JiaXGXiang GaoSZShuming Zhang

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Overview

Dynamic regulation of DNA replication stalling and reactivation in replisome, indicating the role of torsion.

Key Points

  • The study aims to understand how torsional stress affects replisome movement during DNA replication.
  • Developed a high-resolution, real-time assay to monitor replisome movement under torsion.
  • Visualized replisome rotation of DNA during replication.
  • Examined the interaction between helicase and DNA polymerase.
  • Replisome generates ∼22 pN·nm of torque before stalling, twice that of E. coli RNA polymerase.
  • Prolonged torsion-induced stalling inactivates the replisome, while excess DNAP aids replication restart.
  • Gyrase's timely arrival is crucial for resuming replication.
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Cite This Study

Jia et al. (2026) studied this question.

synapsesocial.com/papers/69990de85b97ab4c14ac289ehttps://doi.org/10.1016/j.bpj.2025.11.2634
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