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February 22, 2026Pathogens0 citationsOpen Access

Genomic and Clinical Profile of Hypervirulent Klebsiella pneumoniae (ST23-K1) Liver Abscesses: Experience with Patients at a Romanian University Hospital

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DLDragoș Ștefan LazărENElena NeduANAndrei Nanu

Key Points

  • To characterize the epidemiological, clinical, and genomic profiles of hvKp liver abscesses in Romania.
  • Analyzed 15 cases of hvKp liver abscesses in a tertiary hospital.
  • Conducted genomic sequencing on isolates to identify virulence determinants.
  • Performed cgMLST analysis to assess genetic diversity among strains.
  • Cohort predominantly male with high rates of type 2 diabetes.
  • 93.3% of isolates retained a wild-type susceptible profile.
  • A single ESBL-positive case highlighted resistance risk.
  • Genomic sequencing confirmed the ST23-K1 lineage with key virulence factors.
  • Findings suggest sporadic community acquisition.

Abstract

Introduction: Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging global pathogen that causes severe liver abscesses and metastatic infections. Despite rising concerns regarding multidrug-resistant convergence, molecular data in Romania remain limited. This study characterizes the epidemiological, clinical, and genomic profiles of hvKp liver abscesses in a tertiary hospital, aiming to describe the local virulence landscape and inform clinical management. Results: This study characterizes 15 cases of hvKp liver abscesses in a tertiary hospital. The cohort was predominantly male, with high rates of type 2 diabetes. Although clinical presentation was severe, featuring complications like endogenous endophthalmitis, the outcomes following prolonged antibiotic therapy were generally favorable. Phenotypically, 93.3% of isolates retained a wild-type susceptible profile, while a single ESBL-positive case highlighted the risk of resistance convergence. Genomic sequencing confirmed the presence of the ST23-K1 pandemic lineage carrying key virulence determinants (rmpA, iuc, and peg-344). Crucially, cgMLST analysis revealed genetic heterogeneity, suggesting sporadic community acquisition rather than a clonal nosocomial outbreak. Conclusions: These findings represent the first detailed molecular description of hvKp in Romania, confirming the local circulation of high-risk ST23 clones and underscoring the necessity for early detection and surveillance.

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Cite This Study

Lazăr et al. (2026) studied this question.

synapsesocial.com/papers/699a9d8e482488d673cd3777https://doi.org/10.3390/pathogens15020237
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