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February 25, 2026Analytical Methods0 citations

Cas13a/crRNA trans-cleavage triggered primer exchange reaction based self-priming chain extension for sensitive and label-free infantile pneumonia related microRNA analysis

JYJia YangQHQiuling Huang

Key Points

  • The research aims to develop a sensitive and label-free technique for detecting microRNA related to infantile pneumonia.
  • Utilized cas13a/crRNA complexes for trans-cleavage activation.
  • Developed a primer exchange reaction for chain extension.
  • Evaluated sensitivity and specificity in microRNA detection.
  • Achieved high sensitivity in detecting microRNA.
  • Demonstrated successful label-free analysis.
  • Indicated reduced dependence on conventional amplification methods.

Abstract

Accurate and sensitive detection of microRNA (miRNA) is crucial for both pathophysiological studies and clinical diagnostics. Conventional amplification methods often face limitations such as dependence on thermal cycling, susceptibility to...

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Cite This Study

Yang et al. (2026) studied this question.

synapsesocial.com/papers/699e927bf5123be5ed0503fbhttps://doi.org/10.1039/d6ay00090h
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