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February 27, 20260 citationsOpen Access

CRISPR-Cas Mediated Editing of Susceptibility (S) Genes for Broad-Spectrum Disease Resistance

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USUsha Verma Subhash Sharma

Key Points

  • The aim is to explore the editing of susceptibility (S) genes in plants to confer broad-spectrum disease resistance.
  • Utilized CRISPR technology for precise editing of S genes.
  • Modified plant genetic features that are essential for pathogen infection.
  • Conducted assessments of fitness costs and pathogen adaptation concerns.
  • Demonstrated effective gene disruptions against various pathogens including fungal, bacterial, and viral types.
  • Highlighted the potential for stable and eco-friendly plant resistance traits.
  • Confirmed that integrating CRISPR with genomic knowledge enhances disease-resistant crop design.

Abstract

AbstractSusceptibility (S) genes are host genes that pathogens rely on to infect and multiplywithin plant tissues. Unlike resistance genes, which activate defense responses, S-genessupport processes that microbes exploit. With CRISPR technologies, these genes can nowbe precisely modified through gene disruption, promoter changes, or subtle nucleotide editsto block pathogen compatibility while reducing negative consequences for plant growth.CRISPR tools have shown effectiveness against a wide range of pathogens fungal, bacterial,viral, and nematode by targeting plant features essential for infection. Editing can disrupthost molecules that pathogens depend on or modify interaction points needed for diseasedevelopment. Although there are concerns such as potential fitness costs or pathogenadaptation, modifying S-genes continues to be one of the most promising strategies forproducing stable, eco-friendly, and combinable plant resistance traits. Overall, theintegration of CRISPR technologies with advanced genomic knowledge provides anefficient platform for designing next-generation disease-resistant crops and transformingmodern plant breeding.

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Cite This Study

Usha Verma Subhash Sharma (2026) studied this question.

synapsesocial.com/papers/69a13571ed1d949a99abf59ahttps://doi.org/10.5281/zenodo.18767247
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