Introduction Accurate counting and spatial localization of soybean seeds—particularly Seeds Per Plant (SPP) —are critical for yield estimation and cultivar evaluation. In field environments, however, complex backgrounds, pod occlusion, and uneven grain filling make high-precision counting challenging, and traditional methods often struggle to balance accuracy and robustness. Methods To address these challenges, this study proposes SoyCountNet, a deep learning framework for automatic soybean seed counting and localization at the single-plant level under field conditions. The model is built on a self-constructed field-based phenotyping platform and optimized using the lightweight Point-to-Point Network (P2PNet). For feature extraction, a VGG19BN backbone and a Super Token Sampling Vision Transformer (SViT) module are employed to enhance local feature representation and global contextual understanding. During feature fusion, the Efficient Channel Attention (ECA) mechanism strengthens seed-related features while suppressing interference from leaves, stems, and soil. Furthermore, an improved loss function that combines point-distance constraints with overlap penalties enhances both counting precision and spatial consistency. Results Experimental results demonstrate that SoyCountNet outperforms existing approaches on the field soybean dataset. It achieves a mean absolute error (MAE) of 4. 61, a root mean square error (RMSE) of 6. 03, and a coefficient of determination (R²) of 0. 94. The model demonstrates consistent performance across the tested soybean cultivars, providing reliable SPP estimates within the evaluated dataset. Discussion These findings indicate that SoyCountNet offers a reliable and scalable solution for precise soybean seed counting and localization in complex field environments. Its lightweight architecture allows deployment on intelligent agricultural platforms, supporting high-throughput phenotyping, yield prediction, and precision breeding, while providing a foundation for the future development of intelligent and sustainable agricultural technologies.
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Fei Liu
Qingdao Agricultural University
Qiong Wu
Qingdao Agricultural University
Haoyu Wang
Qingdao Agricultural University
SHILAP Revista de lepidopterología
Frontiers in Plant Science
China University of Petroleum, East China
Qingdao Agricultural University
Institute of Software
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Liu et al. (Wed,) studied this question.
synapsesocial.com/papers/69a285aa0a974eb0d3c00a3a — DOI: https://doi.org/10.3389/fpls.2026.1743104