A pre-synthetic conjugation methodology of DNA and RNA: Combining on-column conjugation and tandem oligonucleotide synthesis

Key Points

• To develop a cost-effective conjugation methodology for DNA and RNA that facilitates bioconjugation applications.
• Described a streamlined strategy for on-column conjugation of nucleic acids.
• Utilized amino modifiers for nucleic acid bioconjugation.
• Integrated tandem oligonucleotide synthesis into the methodology.
• Demonstrated efficiency in the conjugation process.
• Indicated potential for broader applications in bioconjugation.
• Provided a cost-effective solution for lab practices.