An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

Key Points

• To develop a fluorescent sensor for detecting APE1 activity, crucial for breast cancer diagnosis.
• Designed a fluorescent nanosensor using FRET technology.
• Incorporated magnetic beads to enhance detection sensitivity.
• Tested the sensor in breast cancer cell lysates.
• Successfully detected APE1 activity with high sensitivity.
• Demonstrated the 'off-on' mechanism of the fluorescent sensor.
• Indicated potential applications for breast cancer biomarker detection.