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February 28, 2026Journal of Indian Society of Pedodontics and Preventive Dentistry0 citationsOpen Access

Evaluation of salivary pH, buffering capacity, and total antioxidant capacity in children with sleep disorders

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IGIsha GoreVSVabitha ShettyMKM. K. Praveena Kumari

Key Points

  • The research aims to evaluate salivary parameters in children with and without sleep disorders, focusing on pH, buffering capacity, and antioxidant capacity.
  • Conducted a cross-sectional study with 56 children aged 6-11 years.
  • Divided participants into study (sleep problems) and control (no sleep problems) groups.
  • Collected unstimulated morning saliva samples for analysis.
  • Measured salivary pH and buffering capacity using digital pH meter and acid challenge assay.
  • Estimated total antioxidant capacity using the modified phosphomolybdenum spectrophotometric assay.
  • No significant differences in median salivary pH, buffering capacity, and antioxidant capacity between groups (P > 0.05).
  • Effect size calculations showed negligible to small effects for pH and buffering capacity, and a moderate effect for total antioxidant capacity.
  • Children with sleep disorders showed a trend toward lower values in salivary parameters compared to controls.

Abstract

Background: Saliva offers a promising medium for assessing systemic health, including oxidative stress. Investigating the relationship between salivary total antioxidant capacity (TAC) and sleep disordered breathing (SDB) may be crucial for understanding and assessing the impact of SDB in children. Aim: This study aims to evaluate and compare physiological parameters of salivary pH, buffering capacity, and TAC in children with and without sleep disorders. Settings and Design: A cross-sectional study was conducted on 56 children (aged 6–11 years) visiting the Department of Pediatric and Preventive Dentistry. Participants were divided into a study group ( n = 28) with a history of sleep problems and skeletal Class II malocclusion, and a control group ( n = 28) without sleep problems and with normal skeletal relations. Materials and Methods: Unstimulated saliva samples were collected in the morning. Salivary pH and buffering capacity were determined using a digital pH meter and an acid challenge assay. Salivary TAC was estimated using the modified phosphomolybdenum spectrophotometric assay. Statistical Analysis: The statistical analysis was conducted using the Shapiro–Wilk test to assess normality of data and the Mann–Whitney U -test for intergroup comparisons. Effect sizes were calculated using Cohen’s d . Results: Intergroup comparison revealed that the median salivary pH, buffering capacity, and TAC did not differ significantly between the study and control group ( P > 0.05 for all). Effect size calculation using Cohen’s d showed negligible to small effects for pH and buffering capacity, and a moderate effect for TAC, indicating limited clinical differences. Conclusion: Children with sleep disorders showed a trend toward lower salivary pH, buffering capacity, and TAC values than children without sleep disorders. Saliva remains a promising, noninvasive medium for assessing the physiological changes associated with obstructive SDB in children.

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Cite This Study

Gore et al. (2026) studied this question.

synapsesocial.com/papers/69a288590a974eb0d3c04262https://doi.org/10.4103/jisppd.jisppd_530_25
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