Objective: Pseudomonas aeruginosa is a major opportunistic pathogen causing serious hospital-acquired infections, particularly in immunocompromised patients. Its pathogenicity is linked to multiple virulence factors facilitating host invasion and immune evasion. This study aimed to determine the virulence factor profiles of clinical P. aeruginosa isolates and to assess their distribution according to sample type, patient demographics, and clinical units. Materials and Methods: A prospective study was conducted between November 2023 and March 2024, including 100 P. aeruginosa strains isolated from urine, endotracheal aspirates, abscesses, sputum, blood, bronchoalveolar lavage, and ear swab samples. Ten virulence factors (elastase, protease, motility, pyoverdine, pyocyanin, β-haemolysis, twitching motility, mucoid phenotype, catalase, and lipase) were assessed by standard phenotypic methods. Demographic and clinical data were obtained from the hospital automation system. Results: Among the patients with P. aeruginosa-positive cultures, 58% were male and 42% were female (p0.05). Whereas, 65% of the patients were over 60 years old, which showed a significant (p
Çolak et al. (Sat,) studied this question.