Background: This study explores the eco-friendly synthesis of silver nanoparticles (AgNPs) using Mangifera indica leaf extract as a diminishing and settling agent. These AgNPs are notable for their antimicrobial activity. Objective: This study's objective is to produce silver nanoparticles using mango leaf concentrate and assess the antimicrobial efficacy of biosynthesized silver nanoparticles against antibioticresistant bacteria. Method: Comprehensive analysis techniques, including UV-visible spectroscopy, FTIR, DLS, XRD, and TEM, were employed to characterize the properties of the biosynthesized AgNPs. DPPH and BSLA assays were done to measure the antioxidant and cytotoxicity levels. The Kirby Bauer Disc diffusion method was incorporated to analyze the bacterial MIC and zone of inhibition. Result: AgNPs were produced using the green synthesis method. UV results confirmed the presence of SPR at 427nm at 1:70 ratios. TEM results demonstrated that these nanoparticles are spherical. DLS result showed an average particle size of about 41.02nm. FTIR and XRD analysis provide strong evidence of the synthesis of silver nanoparticles with a crystalline structure using mango leaf extract. The silver nanoparticles produced exhibited less cytotoxicity with maximum antioxidant activity at 200μg/mL. AgNPs had strong inhibitory effects on three strains of antibiotic- resistant bacteria: Bacillus cereus, Staphylococcus aureus, and Escherichia coli. Notably, the disc diffusion method revealed that the AgNPs generated the maximum and minimum inhibitory zone of Bacillus cereus at 16 and 8mm at 64 and 8 μg/mL, respectively. Conclusion: This research indicates that silver nanoparticles synthesized from mango leaves may serve as effective antimicrobial agents, especially against antibiotic-resistant pathogenic bacteria.
Jahan et al. (Fri,) studied this question.