Cascade biocatalysis for pyridoxal 5′-phosphate synthesis with ATP autonomy via polyphosphate kinase

Key Points

• The synthesis of pyridoxal 5′-phosphate is achieved efficiently through biocatalytic processes, enhancing productivity.
• Using polyphosphate kinase allows for ATP autonomy during synthesis, making the process more sustainable.
• The approach involves cascade reactions that simplify the overall synthesis pathway, reducing the need for multiple enzymes.
• These findings may enable more cost-effective production of pyridoxal 5′-phosphate in industrial applications.