Utility of flow cytometric PAX5 protein detection for the diagnosis of B-cell acute lymphoblastic leukemia

Key Points

• nPAX5 achieved 100% agreement with traditional immunohistochemistry methods, confirming its reliability.
• The diagnostic performance of nPAX5 shows an AUC of 0.955 with a sensitivity of 91.06%, making it highly effective.
• Utilizing flow cytometry allows for rapid B-lineage assignment in acute leukemia diagnostics.
• These findings support the integration of nPAX5 as a standard marker in clinical practice for diagnosing B-cell malignancies.

Abstract