Red and blue microspheres based immunoassay combined with RPA and Cas12a for dual-detection of nucleic acid from foodborne pathogens

Key Points

• Nucleic acid detection successfully achieved using an immunoassay combining RPA and Cas12a, enhancing sensitivity.
• The method utilized specific red and blue microspheres for dual detection of pathogens, proving its versatility.
• Employing a rapid amplification technique, the approach streamlines the identification of foodborne threats efficiently.
• Potential public health implications exist in rapidly identifying harmful pathogens in food items, thereby preventing outbreaks.