Hybrid Nanofibers for Multimodal Accelerated Wound Healing

Wound healing is a complex physiological process that demands multifunctional therapeutic approaches to ensure effective recovery. This study presents a straightforward approach using blend electrospinning to produce multimodal hybrid nanomaterials that accelerate the wound healing process. Poly(L-lactide-co-ε-caprolactone) (PLCL), cellulose acetate (CA), and polyethylene oxide (PEO) were utilized as biodegradable, compatible, and compliant polymers for generating nanofibers. Hybrid nanofibers functionalized with dexamethasone, ascorbic acid, and hyperbranched polymers introduce anti-inflammatory, regenerative, and antimicrobial properties. Pristine nanofibers with diameters of 0.818 ± 0.028 and 0.845 ± 0.039 µm were generated, while drug-loaded fibers with average diameters of 1.075 ± 0.055 and 1.235 ± 0.075 µm were obtained. The fibers demonstrated a porosity ranging from 72 % to 86 %. Further, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), thermogravimetric analysis (TGA), and contact angle, as well as zeta potential measurements, highlight the physicochemical properties of the fibers. In vivo studies of the nanofibers demonstrated that by day 11, there was a significant acceleration in wound healing. A remarkable acceleration was observed in cell proliferation, granulation, and remodeling phases. The findings emphasize the potential of multimodal hybrid nanofibers as advanced wound dressings and the importance of integrative strategies in wound care.