3D‐Printed Coil Immobilized Enzyme Reactors for Laccase Biocatalysis in Single‐ and Two‐Phase Flow

Abstract Biocatalytic reactions offer mild process conditions and enable production routes based on renewable feedstocks. However, enzymes or microorganisms must be retained in the reactor for high productivity and economic success. Immobilized enzyme reactors (IMERs) were produced by three‐dimensional (3D) printing and functionalized with Trametes versicolor laccase using (3‐aminopropyl)triethoxysilane (APTES) silanization and glutaraldehyde coupling. Kinetic analysis revealed reduced catalytic rates and increased Michaelis coefficients compared to free laccase, whereas thermal and pH stability improved. Two‐phase Taylor flow enhanced substrate conversion, and a coiled flow inverter (CFI) further promoted mass transfer. IMERs maintained substantial activity after storage and repeated use, demonstrating their potential for continuous‐flow biocatalysis.