Functionalized carbon nanotube-based affinity chromatography for polyphenol oxidase purification from different sources and evaluation of kinetic properties

Key Points

• Polyphenol oxidase purification achieved by utilizing functionalized carbon nanotubes, enhancing yield.
• The study reveals a significant increase in enzyme activity associated with purified polyphenol oxidase, reaching 85%.
• Assessment using affinity chromatography demonstrates effective purification techniques for target enzymes.
• These findings indicate potential advancements in enzyme extraction methods, emphasizing the need for robust purification strategies.