Comprehensive UPLC–MS/MS analysis of etomidate and its structural analogs with isomer separation: validation and application to authentic urine samples

Key Points

• Isomer separation confirmed efficient detection of etomidate, allowing accurate analysis.
• Key evidence indicates effective analysis during urine sample testing with known structural analogs.
• Assessment using UPLC–MS/MS method provides rigorous validation for urine sample applications.
• Highlights potential improvements in drug testing protocols for substances like etomidate and its analogs.