Optimization of operational conditions for the production and purification of the alkane hydroxylase enzyme for Bioremediation of Petroleum Waste

Key Points

• Alkane hydroxylase optimization enhances enzymatic purification efficiency, improving bioremediation outcomes.
• Enzymatic production showed a significant increase, achieving a yield increase of 35% under optimized conditions.
• Assessment using enzymatic purification processes highlighted key operational parameters affecting efficacy in petroleum waste treatment.
• Findings support the use of optimized conditions for environmental cleanup strategies, with a focus on reducing petroleum pollution.