A turn-on CRISPR/Cas12a strategy featuring a sterically-hindered activator for in situ fluorescence imaging of H2O2 in vivo

Key Points

• Fluorescence imaging shows improved sensitivity for detecting hydrogen peroxide with the CRISPR/Cas12a approach,
• A notable increase in detection capability was observed, indicating a potential for real-time imaging techniques.
• The method utilizes a turn-on CRISPR/Cas12a strategy integrated with a sterically-hindered activator for enhanced results.
• This approach highlights significant advancements in in vivo imaging; further studies are needed for clinical applications.