Development of a TaqMan-based triplex qPCR assay for the simultaneous detection of Muscovy reovirus, novel duck reovirus, and Riemerella anatipestifer

In summary, the TaqMan-based triplex qPCR detection assay established in this study can be used as a fast, sensitive and reliable technical means for large-scale monitoring of RA, MDRV and NDRV infection; at the same time, the analysis of the supporting drug-resistant gene detection assay can provide reference for the rational use of antibacterial drugs in duck breeding production. Test the basis, and then help the breeding link to take scientific and effective prevention and control intervention measures in a timely manner.