Bioinformatics-assisted mining and engineering of α-amylase for efficient hydrolysis of raw corn and microalgal starch

Key Points

• Enhanced hydrolysis efficiency was achieved using bioinformatics-assisted engineered α-amylase, impacting starch processing.
• The study showed a significant increase in starch breakdown, achieving up to 85% hydrolysis in specific conditions.
• Bioinformatics tools helped in the design of α-amylase variants, leading to improvements over native enzymes.
• These findings highlight the potential for using bioinformatics in enzyme engineering to optimize industrial applications.