Hairpin-locked modular polymerase/nickase cycle amplification enables ultrasensitive urinary miRNA detection for acute kidney injury diagnosis

Key Points

• The detection method significantly improves diagnosis for acute kidney injury with high sensitivity.
• Ultrasensitivity is achieved through a polymerase/nickase amplification technique, allowing better biomarker identification.
• Hairpin-locked modular amplification was utilized for improved accuracy in miRNA detection, enhancing diagnostic potential.
• These findings suggest that the new technique could facilitate earlier identification of acute kidney injury in clinical settings.