An optimized method for establishing experimental rat periodontitis using “double-ligature” technique

Key Points

• The double-ligature technique effectively induces periodontitis, resulting in noticeable alveolar bone loss.
• Rats showed significant inflammatory responses over the study duration, enhancing the model's utility for dental research.
• Analysis involved observations in various rat groups, ensuring a robust experimental setup with consistent parameters.
• Such an optimized method may enable better understanding of periodontal disease mechanisms and treatment approaches.