Development and validation of a green HPTLC method for liquiritin and glabridin: Application to nanoemulsion formulations and plasma quantification

Key Points

• A validated green HPTLC method quantifies liquiritin and glabridin efficiently in nanoemulsions.
• Experiments show high accuracy with a recovery rate of 98% for both compounds, under 0.01 mg/mL detection limits.
• Assessment using high-performance thin layer chromatography allows effective analysis of formulation stability.
• This approach may enhance plant-based formulation techniques, while further studies are required for broader applicability.