Development of a high-throughput sensitive SB-PCR assay for rapid detection of laboratory-escaped antibiotic resistance genes

Key Points

• The assay enables rapid detection of antibiotic resistance genes in laboratory settings, ensuring effective monitoring.
• Sensitivity of the SB-PCR assay was validated against various antibiotic resistance gene samples, enhancing accuracy.
• High-throughput capabilities allow for simultaneous testing of multiple samples, streamlining the detection process.
• Protecting public health requires prompt identification of antibiotic resistance to inform treatment decisions.