First report of CRISPR/Cas13a-based rapid detection of groundnut bud necrosis virus without amplification

Key Points

• Rapid detection of groundnut bud necrosis virus is possible without amplification, leading to faster diagnostics.
• The detection method utilizes CRISPR/Cas13a technology, providing a novel approach to virology.
• Assessment using a CRISPR/Cas13a-based approach indicates high specificity and sensitivity.
• The findings support the potential for effective field applications in agricultural virus management.