Structure-initiated CHA variant coordinating SDA for cascade amplification in CRISPR/Cas12a-based miRNA analysis

Key Points

• Enhanced cascade amplification significantly improves miRNA detection accuracy.
• A notable increase in sensitivity was observed, with detection thresholds down to 1 fM.
• Analysis utilized a novel structure-initiated variant designed for CRISPR/Cas12a systems.
• This approach highlights potential for more effective RNA analysis in various applications.