There is increasing evidence that crop production practices can engage plant protection measures to improve crop yields and control pests/pathogens together (El-Nagdi et al., 2019;Abd-Elgawad et al., 2024). Vimala et al. confirmed that organic nutrient sources and biocontrol agents (BCAs) can severely affect the root-knot nematode (RKN) Meloidogyne incognita. The authors explored the impact of Mentha spicata and Piper longum essential oils and extracts, along with Bacillus amyloliquefaciens and B. subtilis as well as organic amendments on M. incognita infecting tomato plants. While essential oils showed significant nematode-juvenile mortality and egg hatching inhibition, all treatments, comprising their combinations with organic amendments (farmyard manure, vermicompost, and paddy straw), could significantly reduce nematode populations, enhance tomato growth, and boost soil fertility. Such combinations performed comparably to the nematicide Velum® Prime 400 SC. The results also showed a significant increase of soil organic carbon and NPK content (P < 0.05), demonstrating that these treatments could provide reliable, eco-friendly alternatives for M. incognita control, contributing to resilient/sustainable tomato production systems. Lillo et al. study evaluated the efficacy of six fertilization treatments, adjusted to provide equivalent units of N-fertilization, on M. incognita-infested greenhouse cucumber. Interestingly, the fresh chicken manure treatment yielded the highest cucumber production, despite insignificant differences in RKN-disease severity between treatments. Yet, the treatment was the most effective in minimizing RKN abundances in soil, followed by pelletized chicken manure, fresh cow manure, and finally composted cow manure. Contrary to organic amendments that enhanced the complexity of the soil food web, fast-release inorganic fertilizers led to its degradation and simplification. Inorganic fertilizers were the least effective in decreasing the nematode abundance. Notably, cucumber cultivation and fertigation throughout the crop cycle enriched the soil with nutrients, intensified the bacteria-dominated organic matter degradation channel, and further simplified the soil food web, in addition to suppressing RKN disease.Könker et al. focused on the use of Pochonia chlamydosporia to synergistically reinforce systemic plant defense reaction of Phacelia tanacetifolia against Meloidogyne hapla. Transcriptome and metabolome analysis of plant leaves revealed that the metabolome was quite stable except for the first two days. Comparing P. chlamydosporia singly with M. hapla + P. chlamydosporia treatment manifested larger impacts after 6 compared to 2 days, aligning with the later root infestation by P. chlamydosporia compared to M. hapla. Shifts in transcripts and metabolites were higher in the combined treatment relative to the single inoculum which support the conclusion that P. chlamydosporia induces plant defense in a distinguished and beneficial manner when combined with M. hapla. Their results tentatively proposed that P. chlamydosporia application against M. hapla can be more effective via backing the underlying tritrophic interactions with specific additives, e.g. phytohormones or amino acids. This study provided valuable insights into the systemic response of plants to PPNs and a biocontrol fungus.The study of Kisaakye et al. addressed chitin-enriched insect frass fertilizers as cheap and safe biorational alternatives for managing RKN (M. incognita). The authors tested seven chitin-fortified black soldier fly frass fertilizer extracts (chFE) for their suppressing of M. incognita and effects on spinach growth relative to a chemical nematicide, Velum ® Prime. Black solder fly (BSF) frass and BSF exuviae, merged in varying proportions to form seven compounds, were mixed with effective microorganisms, biochar and molasses, in a fermentation process to obtain chFE. Meloidogyne incognita-J2s paralysis reached 100% when exposed to either chFE or Velum ® Prime. Further, the J2 attained mortality using chFE (95%) was comparable to the value achieved using Velum ® Prime (96%). In all treatments, mortality increased with exposure time. Up to 85% suppression of nematode-gall development was achieved when spinach plants were grown in soil drenched with chFE. Suppressions of other RKN growth parameters were obtained. Also, chFE application significantly increased spinach root and shoot biomass considerably, compared to the commercial nematicide. These findings demonstrated the nematicidal potential of chFE and its benefits on crop production as a promising multipurpose, regenerative, and cost-effective input for PPN-sustainable management and boosting crop yield.Grasping the role of grafting for PPN resistance in plants is essential in devising adequate management solutions. Following M. incognita infection, Li et al. found that the grafted progeny GHF1, F1 progeny of tobacco grafts 'G278 (resistant rootstock) + Honghua Dajinyuan (HD, susceptible scion), showed notably enhanced resistance compared to its scion HD. This was characterized by raised chlorophyll levels, increased activity of phenylpropanoid metabolic enzymes and disease-related proteins, reduced membrane lipid peroxidation, and stable antioxidant enzyme levels. Among all combinations tested, GHF1 displayed the most robust resistance phenotype, stressing its potential as a superior germplasm resource. The study offers a sound framework for assessing the agronomic traits and stress responses of graft-derived tobacco progeny under PPN pressure by integrating phenotypic, physiological, and biochemical analyses.Likewise, assessing the accumulation of phyto-nematicidal cytotoxicity is necessary for developing appropriate designs of safe and sustainable strategies. Mashela et al. evaluated the toxicity of chemical residues of Nemarioc-AL and Nemafric-BL phytonematicides in tissues of tomato planted in different soil types. Cucurbitacins A and B were present in both phytonematicides. Accumulation of their residues in tomato tissues was independent of the treated soil type and type of phytonematicide applied to the soil. Such toxic compounds can cause severe illness in humans if ingested in large amounts, but the products can be used at lower concentrations to manage PPNs and avoid their toxicity. The authors stressed that farmers must use the non-toxic doses recommended for the safe use of both products, with negligible toxicity.
Abd-Elgawad et al. (Fri,) studied this question.