Genetic material of the cell in interphase nucleus is present in a form of a dense DNA-protein structure named chromatin. Structure and dynamics of the single nucleosome, which is the basic unit of DNA compactization, is currently well-studied, although data about the structural and functional organization of higher-level chromatin folding are still scarce. In the present work, a method for visualizing of polynucleosomal constructs using atomic force microscopy is proposed. Polynucleosome assembly on a plasmid with the use of recombinant histone octamers was demonstrated. It was established that glutaraldehyde treatment of a polynucleosome sample before its immobilization on a surface prevents nucleosome disassembly, and their height and width corresponds well with previously obtained data. Plasmids themselves were predominantly in extended conformation, which may help study DNA-protein interactions.
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A. V. Lyubitelev
D. V. Bagrov
O. V. Geraskina
Moscow University Biological Sciences Bulletin
Lomonosov Moscow State University
Fox Chase Cancer Center
Institute of Gene Biology
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Lyubitelev et al. (Mon,) studied this question.
www.synapsesocial.com/papers/69a7cc4cd48f933b5eed7dfd — DOI: https://doi.org/10.3103/s0096392525600954