An improved throughput workflow to identify ion transport and membrane lysis agents for antimicrobial discovery

Key Points

• The central aim is to enhance the identification of ion transport and membrane lysis agents for antimicrobial applications.
• Developed a new workflow to analyze small molecule ion transporters.
• Evaluated ion transport efficiency and membrane lysis effects in microbial models.
• Employed quantitative assays to assess the impact on microbial growth.
• Demonstrated improved throughput in identifying effective ion transport agents.
• Showed potential candidates that significantly affect microbial membrane integrity.
• Identified several small molecules with dual action on ion transport and lysis.