In vitro study evaluates chitosan nanoparticles' role in enhancing antimicrobial efficacy against E. faecalis and C. albicans, suggesting improved treatment options.
Key Points
To evaluate the effectiveness of chitosan nanoparticles in enhancing antimicrobial agents against persistent endodontic infections.
In vitro agar well-diffusion model with four formulations: calcium hydroxide, CNPs with calcium hydroxide, 2% chlorhexidine, and CNPs with chlorhexidine.
Evaluation of antimicrobial efficacy against Enterococcus faecalis and Candida albicans at 3- and 7-day intervals.
Statistical analysis using one-way ANOVA and Tukey’s post hoc test for significance.
Incorporation of chitosan nanoparticles significantly enhanced antimicrobial activity.
Chlorhexidine with CNPs resulted in the largest inhibition zones against C. albicans (28.0 ± 1.9 mm, P = 0.042).
Calcium hydroxide with CNPs exhibited a time-dependent increase in efficacy against E. faecalis (from 0 to 23.0 ± 2.1 mm, P = 0.003).