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March 6, 2026American Journal of Tropical Medicine and Hygiene0 citations

Detection of Multiple Dengue Virus Serotypes Circulating in Somalia

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TTTitus TugumeBEBernard ErimaGMGraham Matulis

Key Points

  • The aim is to identify circulating dengue virus serotypes in Somalia through sample screening.
  • Collected 374 samples from patients with fever under an acute febrile illness surveillance protocol.
  • Screened samples for dengue virus serotypes using real-time reverse transcription polymerase chain reaction.
  • Conducted phylogenetic analysis on positive samples.
  • Identified 55 samples with dengue virus RNA, comprising 14.7% of total samples.
  • Detected three dengue serotypes: DENV-1 (n = 24), DENV-2 (n = 21), and DENV-3 (n = 12).
  • Discovered two cases of coinfection between DENV-1 and DENV-2, and DENV-2 and DENV-3.

Abstract

Dengue virus (DENV) is widely distributed across Africa but underreported due to limited surveillance and diagnostic capacity. Under an acute febrile illness surveillance protocol, we collected and screened 374 samples from patients with fever in Mogadishu, Somalia. Samples were screened for DENV serotypes using real-time reverse transcription polymerase chain reaction, and positive samples were sequenced and phylogenetically analyzed. We identified 55 samples containing orthoflavivirus RNA (14.7%). Of these 55 samples, three dengue serotypes, DENV serotype 1 (DENV-1, n = 24), DENV serotype 2 (DENV-2, n = 21), and DENV serotype 3 (DENV-3, n = 12), were identified. Two cases of coinfections were detected: 1) between DENV-1 and DENV-2 and 2) between DENV-2 and DENV-3. DENV-1 had two genetic clusters, both related to lineages from Asia. DENV-2 grouped within a set of sequences from Asia and Africa, particularly close to a DENV-2 outbreak in Kenya. The DENV-3 grouped with isolates from Oceania Asia, clustering with a case of dengue fever identified in Italy in 2023 and Ethiopia in 2023. Our findings suggest concurrent infections of the three serotypes with DENV-3 being more widely spread in East Africa.

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Cite This Study

Tugume et al. (2026) studied this question.

synapsesocial.com/papers/69aa6f3c531e4c4a9ff5958ehttps://doi.org/10.4269/ajtmh.25-0445
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