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March 6, 2026Stem Cell Research0 citationsOpen Access

Generation of an induced pluripotent stem cell line from a long QT syndrome type 2 patient carrying the pathogenic KCNH2 c.1682C>T (p.Ala561Val) variant

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MDMarlon DeBoseDDDingqian DingAGAnna G. Griggs

Key Result

An iPSC line with the pathogenic KCNH2 c.1682C>T variant was generated, retaining pluripotency and differentiation potential, enabling LQT2 patient-specific research.

Key Points

  • The study aims to generate a viable iPSC line from an LQT2 patient to explore potential therapeutic applications.
  • Generated iPSC line from a long QT syndrome type 2 patient carrying KCNH2 variant.
  • Assessed karyotype and stem cell morphology of iPSCs.
  • Evaluated pluripotency and differentiation capabilities.
  • iPSC line showed a normal karyotype and typical stem cell morphology.
  • Demonstrated pluripotency and the ability to differentiate into three lineages.
  • Provides a resource for developing translational and precision medicine strategies.

Structured PICO

P
Population
Induced pluripotent stem cell (iPSC) line generated from a patient with Long QT syndrome type 2 (LQT2) carrying the pathogenic KCNH2 c.1682C>T (p.Ala561Val) variant who experienced syncope.
I
Intervention
Generation of an induced pluripotent stem cell (iPSC) line
O
Outcome
Generation of an iPSC line exhibiting normal karyotype, typical stem cell morphology, pluripotency, and trilineage differentiation potential

The generation of this patient-specific LQT2 iPSC line provides a valuable resource for disease modeling and precision medicine research in cardiac channelopathies.

Abstract

Long QT syndrome type 2 (LQT2) is a life-threatening cardiac channelopathy caused by variants in the KCNH2 gene, which encodes the hERG channel. Reduced rapid delayed rectifier potassium current (I Kr ) delays ventricular repolarization and prolongs the QT interval, increasing susceptibility to Torsade de Pointes and sudden cardiac death. A LQT2 induced pluripotent stem cell (iPSC) line carrying the pathogenic KCNH2 c.1682C>T (p.Ala561Val) variant was generated from a patient who experienced syncope; the resulting iPSC line exhibits a normal karyotype, typical stem cell morphology, pluripotency, and trilineage differentiation potential, providing a valuable resource for disease- and patient-specific translational and precision medicine research.

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Cite This Study

DeBose et al. (2026) studied this question. An iPSC line with the pathogenic KCNH2 c.1682C>T variant was generated, retaining pluripotency and differentiation potential, enabling LQT2 patient-specific research.

synapsesocial.com/papers/69aa7008531e4c4a9ff59647https://doi.org/10.1016/j.scr.2026.103951
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for modeling cardiac arrhythmias: strengths, challenges and potential solutions2024 · 30 citations
  2. 2Generation of three induced pluripotent stem cell lines from a long QT syndrome type 2 family harboring the pathogenic KCNH2 c.209A > G (p.His70Arg) variant2025 · 2 citations
  3. 3Long QT Syndrome KCNH2 Variant Induces hERG1a/1b Subunit Imbalance in Patient-Specific Induced Pluripotent Stem Cell–Derived Cardiomyocytes2021 · 33 citations
  4. 4Long QT Syndrome Type 2: Emerging Strategies for Correcting Class 2 KCNH2 (hERG) Mutations and Identifying New Patients2020 · 44 citations
  5. 5hiPSC-Derived Cardiomyocyte Model of LQT2 Syndrome Derived from Asymptomatic and Symptomatic Mutation Carriers Reproduces Clinical Differences in Aggregates but Not in Single Cells2020 · 30 citations