The pH-dependence of copper( ii / i ) reduction potentials in variants of P. aeruginosa azurin with surface histidine variations

Key Points

• To examine how the position of histidine residues affects the pH-dependent reduction potentials of azurin variants.
• Investigated different azurin variants with surface histidine mutations.
• Measured pH-dependent reduction potentials of copper ions in these variants.
• Alterations in histidine position significantly impacted the reduction potentials.
• The pH level altered the behavior of these copper-containing proteins.