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March 6, 2026Microbiology Resource Announcements0 citationsOpen Access

Lon protease-mediated secretome profiling in Klebsiella pneumoniae

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CRChelsea ReitzelJGJennifer Geddes-McAlister

Key Points

  • The research investigates how Lon protease affects the protein profiles in the extracellular environment of Klebsiella pneumoniae.
  • Applied bottom-up proteomics to analyze proteins
  • Compared wild-type and Δlon variants of Klebsiella pneumoniae
  • Examined the secretome of both strains
  • Identified distinct protein profiles between wild-type and Δlon strains
  • Highlighted the role of Lon in biofilm formation and antibiotic resistance
  • Revealed insights into iron response and protein transport mechanisms

Abstract

ABSTRACT Lon protease is a global regulator in bacteria affecting biofilm formation, antibiotic resistance, toxin-antitoxin complexes, and proteolysis of damaged proteins. In Klebsiella pneumoniae , Lon has roles in iron response, protein transport, and membrane assembly. Here, we apply bottom-up proteomics to identify proteins in the extracellular environment of WT and Δ lon K. pneumoniae .

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Cite This Study

Reitzel et al. (2026) studied this question.

synapsesocial.com/papers/69aa7066531e4c4a9ff5a183https://doi.org/10.1128/mra.00311-25
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