The expression of tyrosine kinase and threonine promotes the progression of lung adenocarcinoma and is linked to a negative prognosis.

Threonine and tyrosine kinase (TTK) is a crucial element of the spindle assembly checkpoint. This study aimed to investigate the prognostic value of TTK in LUAD and its correlation with immunity. Data from The Cancer Genome Atlas was used in the study to examine TTK expression in both lung adenocarcinoma (LUAD) and normal tissue samples. To investigate the correlation between TTK expression and overall survival, multivariable analysis and Kaplan-Meier survival curves were utilized in this study. The biological function of TTK in LUAD was investigated using LinkedOmics and the STRING database for interacting genes/proteins. Furthermore, examine the relationship between TTK expression and its DNA methylation. Evaluated the correlation between the expression of the TTK gene and the infiltrating levels of 24 different types of immune cells. Additionally, cell culture validation was conducted in A549 cells and BEAS-2B cells. Expression levels of TTK were significantly increased in LUAD samples from the The Cancer Genome Atlas compared to normal tissue samples. TTK plays a role in cell cycle checkpoints, meiotic recombination, and DNA replication. TTK demonstrates a strong functional correlation with 10 partner genes. Furthermore, there is an inverse relationship between TTK DNA methylation and both TTK expression and overall survival rates among LUAD patients. TTK expression has also been found to be associated with immune cell presence, potentially impacting immune cell infiltration and serving as a predictive factor for patient prognosis. Cell culture investigations revealed significantly elevated levels of TTK mRNA and protein expression in A549 cells compared to BEAS-2B cells. TTK expression in LUAD is correlated with disease advancement, unfavorable prognosis, and alterations in the immune cell composition. TTK levels could offer valuable insights into the progression and prognosis of LUAD, as well as potential implications for immunotherapy strategies.