Evaluation of the anti-inflammatory and antioxidant effects of Azadirachta excelsa Jacobs extract via modulation of the NF-κB signaling pathway in RAW264.7 macrophages

Puntos clave

• To evaluate the anti-inflammatory and antioxidant effects of Azadirachta excelsa extract by regulating the NF-κB signaling pathway in macrophages.
• Utilized RAW264.7 macrophage cell line to assess the effects of the extract.
• Administered lipopolysaccharides (LPS) to induce inflammation.
• Measured iNOS and NO production levels.
• Analyzed ROS production and pro-inflammatory cytokine mRNA expression.
• AEE significantly inhibited excessive iNOS and NO production in LPS-treated macrophages.
• AEE reduced ROS production induced by LPS.
• Pro-inflammatory cytokine mRNA expression decreased significantly with AEE treatment.