Laboratory study reveals that Guineenoside B induces apoptosis and inhibits metastasis in prostate cancer cells, suggesting its potential as an anticancer agent.
Key Points
The aim is to evaluate the anticancer potential of Guineenoside B isolated from Piper guineense against prostate cancer.
Isolated three triterpenoid saponins from Piper guineense.
Conducted anti-cell growth, proliferation, and clonogenic assays.
Investigated apoptosis induction and cell migration/invasion.
Assessed key proteins related to cell proliferation and migration.
Guin-B showed significant inhibition of PC3 and LNCaP cell growth with IC50 values of 10 µg/mL and 8 µg/mL, respectively.
Induced nearly 50% increase in apoptotic cell population at low concentrations.
Significant increase in caspase-3 activity was observed.
Inhibited migration and invasion of PC3 cells and enhanced cell adhesion.
Decreased expression of pAKT, AKT, mTOR, and VEGF proteins was measured after treatment.