Complete genome sequence of Sphingomonas sp. gentR, a high-level gentamicin-resistant bacterium

We present the complete genome sequence of Sphingomonas sp. gentR, a strain exhibiting high-level resistance to gentamicin (MIC = 40 mg/mL). The genome was assembled from hybrid Illumina and Nanopore sequencing data into a gap-free sequence of 4.0 Mbp, comprising one chromosome and two plasmids. A total of 3,692 coding sequences were predicted, with comprehensive functional annotation revealing genes associated with antibiotic resistance, stress adaptation, and metabolic diversity. Three confirmed resistance genes—ANT(2″)-Ia, ANT(3″)-IIa, and Sul1—were co-localized within a genomic island on plasmid B. This dataset provides insight into the genetic basis of high-level aminoglycoside resistance in Sphingomonas and serves as a valuable resource for studying horizontal gene transfer, environmental adaptation, and bioremediation potential. The genome sequence is publicly available under GenBank accessions CP144670–CP144672 and China National Genomics Data Center (accession number GWHDOHA00000000).