Curcumin enhances radiosensitivity of head and neck squamous cell carcinoma by regulating the long non-coding RNA MYOSLID

Key Points

• The study aims to determine if curcumin enhances radiosensitivity in head and neck squamous cell carcinoma by regulating MYOSLID.
• Conducted lncRNA transcriptome sequencing to identify key lncRNAs affected by curcumin.
• Divided FaDu cells into control, radiotherapy, curcumin, combination, and MYOSLID overexpression groups.
• Measured MYOSLID expression via qRT-PCR and assessed cell proliferation, invasion, and apoptosis using various assays.
• Analyzed protein expressions related to DNA damage and repair pathways through Western blotting.
• Bioinformatics identified MYOSLID as a key lncRNA downregulated by curcumin.
• Curcumin and radiotherapy reduced MYOSLID expression, with the combination yielding the most significant reduction (P<0.05).
• Both treatments inhibited FaDu cell growth, with the combination showing the strongest effect.
• Mechanistic studies indicated that curcumin and radiotherapy upregulated DNA damage markers, enhancing the effect when combined.
• MYOSLID overexpression partially reversed growth inhibition and modified DNA damage response markers (P<0.05).

Abstract