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March 21, 2026Bioelectromagnetics0 citations

Relative Comparison of the Static Magnetic Field and Oscillating Magnetic Field Effects on Gram‐Negative and Gram‐Positive Bacteria via Evaluating Oxidative Stress and DNA Fragmentation

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HBHatice BaşHÇHatice Kanbur ÇavuşNMNicola Morley

Key Points

  • The aim is to evaluate how static and oscillating magnetic fields affect oxidative stress and DNA fragmentation in two types of bacteria.
  • Bacterial cultures of Staphylococcus aureus and Escherichia coli were exposed to magnetic fields.
  • Magnetic field intensities ranged from 2 to 16 mT at a frequency of 50 Hz for 8 hours.
  • Measurements included optical density, nitric oxide levels, superoxide radicals, and antioxidant enzyme activities.
  • Exposure to both SMF and OMF led to growth inhibition in bacteria as indicated by decreased optical density.
  • Significant increases in oxidative stress markers, such as nitric oxide and superoxide anion radicals, were observed.
  • E. coli showed a greater response compared to S. aureus, especially at higher magnetic field intensities.

Abstract

The effects of static magnetic field (SMF) and oscillating magnetic field (OMF) on oxidative stress responses and DNA fragmentation were investigated in Staphylococcus aureus and Escherichia coli. Bacterial cultures were exposed to magnetic field intensities ranging from 2 to 16 mT at 50 Hz for 8 h. Exposure to both SMF and OMF caused intensity-dependent decreases in optical density, indicating growth inhibition. In parallel, nitric oxide and superoxide anion radical levels, as well as the activities of catalase and superoxide dismutase, increased significantly in a field-strength-dependent manner. Glutathione disulfide levels were enhanced while reduced glutathione values decreased, reflecting a redox imbalance. Moreover, malondialdehyde and DNA fragmentation levels were markedly elevated, confirming oxidative and genotoxic stress. Comparative analysis revealed that E. coli exhibited more pronounced responses than S. aureus, with significant effects emerging above approximately 6-8 mT for OMF and 8-10 mT for SMF exposure. Overall, these findings demonstrate that both OMF and SMF can induce oxidative stress and DNA damage in bacterial cells, with OMF producing stronger effects. The results highlight the differential susceptibility of Gram-positive and Gram-negative bacteria to electromagnetic exposure and suggest potential implications for non-antibiotic antimicrobial strategies based on magnetic fields. Bioelectromagnetics. 00:00-00, 2026. © 2026 © 2026 Bioelectromagnetics Society.

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Cite This Study

Baş et al. (2026) studied this question.

synapsesocial.com/papers/69be35ba6e48c4981c6741fehttps://doi.org/10.1002/bem.70050
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