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March 21, 2026International Journal of Molecular SciencesOpen Access

Revisiting vSGLT: Non-Radioactive Characterization of the Vibrio Na+/Galactose Cotransporter by SURFE2R N1 Solid-Supported Membrane Electrophysiology

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Authors

NENatalia ErmolovaTAThorsten AlthoffEWErnest M. Wright

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Overview

Electrophysiology revisits vSGLT to analyze sodium-coupled sugar transport, suggesting a reliable characterization method.

Key Points

  • To characterize the Vibrio sodium-galactose cotransporter (vSGLT) using non-radioactive methods.
  • Utilized solid-supported membrane electrophysiology with the Nanion SURFE2R N1 system.
  • Analyzed transient currents in proteoliposomes containing purified vSGLT after substrate application.
  • Examined responses with various sugars such as D-galactose, D-glucose, and D-fucose.
  • D-galactose generated the highest Na+-dependent current responses.
  • Kinetic parameters were similar to those found in previous radiolabeled assays, with Km(Na+) ≈ 18 mM and Km(gal) ≈ 9.8 mM.
  • Confirmed that vSGLT maintains its original substrate selectivity and coupling stoichiometry.

Cite This Study

Ermolova et al. (2026) studied this question.

synapsesocial.com/papers/69be35d76e48c4981c6743e9https://doi.org/10.3390/ijms27062790
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