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March 24, 2026Carbohydrate Polymer Technologies and Applications0 citationsOpen Access

Property Control and Functional Expression of Partially Deacetylated Nanochitin with Various Organic Acid Counter Ions: Antibacterial Activity and Hydrogel Formation

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HNHayate NakamineMAMasaaki AkamatsuSIShinsuke Ifuku

Key Points

  • To explore how different organic acid counter ions influence the properties and performance of partially deacetylated nanochitin.
  • Utilized thirteen carboxylic acid derivatives as counter ions.
  • Analyzed dispersion characteristics and nanofibrillation efficiency of nanochitin.
  • Measured zeta potential and optical transmittance of the dispersions.
  • Aliphatic acids enhanced dispersion stability, while aromatic acids hindered nanofibrillation.
  • Nanochitin prepared with acetic acid had a high nanofibrillation yield and specific fiber dimensions.
  • Nanochitin acetate and benzoate showed strong antibacterial activity against E. coli and S. aureus.
  • Nanochitin with acrylic acid formed pH-responsive hydrogels.

Abstract

Cationic partially deacetylated nanochitin exhibits counter-ion-dependent physicochemical properties and nanofibrillation behavior. In this study, the effects of organic acid counter ions on the dispersion characteristics, nanofibrillation efficiency, and functional performance of partially deacetylated nanochitin were systematically investigated to establish rational guidelines for acid selection. Thirteen carboxylic acid derivatives, including aliphatic and aromatic acids, were employed as counter ions. The zeta potential of aqueous nanochitin dispersions strongly depended on the acid species and showed a clear positive correlation with optical transmittance and nanofibrillation yield. Aliphatic carboxylic acids provided higher dispersion stability, whereas aromatic acids tended to suppress nanofibrillation due to intermolecular interactions, which was reflected in nanofiber morphology. Nanochitin prepared using acetic acid exhibited a high nanofibrillation yield with an average fiber width of ∼3 nm and length of ∼430 nm. In addition, nanochitin acetate and benzoate showed enhanced antibacterial activity against Escherichia coli and Staphylococcus aureus. In contrast, nanochitin with acrylic acid underwent gelation through radical polymerization, forming pH-responsive hydrogels via ionic crosslinking with poly(acrylic acid). These findings demonstrate that organic acid counter ions play a crucial role in tailoring the structure–property relationships of partially deacetylated nanochitin.

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Cite This Study

Nakamine et al. (2026) studied this question.

synapsesocial.com/papers/69c2294caeb5a845df0d3861https://doi.org/10.1016/j.carpta.2026.101123
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