Characterizes a hiPSC line expressing a voltage sensor, enabling non-invasive membrane potential monitoring in human cells, suggesting potential for drug screening and developmental...
Key Points
The research aimed to create and characterize a hiPSC line that can express the rEstus voltage sensor for studying membrane potential dynamics.
Generated the UKBi015-B-6 hiPSC line via targeted insertion at the AAVS1 locus.
Developed a doxycycline-inducible expression system for rEstus.
Conducted optical electrophysiology techniques to measure membrane potential.
Successfully induced rEstus expression in hiPSCs using doxycycline.
Enabled non-invasive monitoring of membrane potential dynamics.
Facilitated high-throughput studies across various cell types derived from hiPSCs.