This study evaluated the efficiency of an scFv antibody-based sex-sorting method using HL magnetic microbeads for the separation of X- and Y-chromosome-bearing sperm in Landrace boar semen, as well as its effects on sperm quality parameters. A dose-dependent plateau in H4L4 antibody coupling efficiency was observed, with 2 mg identified as the optimal concentration, consistently achieving approximately 50% sperm-binding efficiency across boars. Sex ratio analysis confirmed effective discrimination between X- and Y-bearing sperm, with the X-enriched fraction showing a high proportion of X-sperm (77–81%) and the Y-enriched fraction exhibiting high Y-sperm purity (>83%). CASA revealed a significant effect of the sex-sorting process on sperm kinematics (p 81%), while a significant reduction in viability was observed in the Y-enriched fraction (50.85%; p 0.05). In conclusion, this study demonstrates that HL magnetic bead-based sperm sexing effectively separates X- and Y-sperm in chilled Landrace boar semen, while preserving the quality of X-enriched sperm.
Satsook et al. (Tue,) studied this question.
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